Materiale S.S. Bioinformatica e Biostatistica
Materials
FCI
Description
R-language algorithm for the statistical analysis of data from real-time PCR experiments for the absolute quantification of nucleic acids.
Reference
Verderio P et al. BMC Bionformatics, 2008; 9:13 (PDF)
Downloads
The FCI.zip folder contains the following files:
- User Guide FCI (Guida_utilizzo_FCI.pdf)
- Example Dataset (example_data.csv)
- R-language code (FCI_1_3.txt)
- R-language command to import, implement and visualize the FCI output (FCI_run.txt)
- Interpretation guide (Guida_interpretazione_risultati.pdf)
- FCI Output (FCI_output.pdf)
- Figure (FCI_figura.pdf)
- Previous version of the algorithm (FCI_1_2.txt, FCI_1_1.txt)
NqA
Description
R-language algorithm useful for the identification of a small number of microRNAs to be used for the normalisation of data from high-throughput qPCR experiments in view of future validation studies.
Reference
Verderio P et al. Analytical Biochemistry, 2014
Downloads
The NqA.zip folder contains the following files:
- User Guide NqA (userguide_NqA.pdf)
- Example Dataset (1.1Inputfile.dat, 1.2Inputfile.dat)
- R-language code (NqA_code.R)
- R-language command to import, implement and visualize the FCI output (NqA_output.pdf)
- NqA Output (NqA_output.pdf)
- Figure (NqA_figura.pdf)